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Localization of other nucleus-resident proteins is not affected by Liar. GFP (A), YFP-ERβ (B), and DsRed–T-bet (C) were not excluded from the nucleus by DsRed-Liar. Fluorescent imaging of Myc-tagged Liar was obtained with anti-Myc followed by the addition of anti-mouse Ig–FITC.
