Fig 4.

ChIP assays at evolutionarily conserved KLF1 and KLF2 binding sites in the promoters of the mouse gene for Myc. The consensus site for KLF1 binding is CCNCNCCC, and that for KLF2 is CCRCCC (CCACCC or CCGCCC). Each tick mark at the bottom of the diagram represents an ECR between the mouse and human genes for Myc. The ECR sequences are designated intergenic, intron, or coding. P1 (promoter 1) of the gene for Myc is at the left and includes the −126 KLF1/KLF2 consensus site, and P2 is to the right and includes the +2074 consensus binding site. These −126 and +2074 binding sites are within ECRs. ChIP assay values obtained with antibodies specific for KLF1 and KLF2 were compared to that obtained with IgG, which was set to 1. **, P = 0.0158; *, P = 0.0405 (unpaired t tests). The arrows indicate transcription start sites.