Fig 5.

(A) KLF1 transactivates the promoters of the gene for Myc in K562 cells. The black bars represent transfections with the luciferase reporter gene only, and the gray bars represent transfections which additionally have the expression plasmid pSG5-mEKLF. pBv-luc is a promoterless luciferase construct. Del-2269 and Del-352 are two different Myc promoter-luciferase fusion constructs. Data are the mean values from at least 3 independent samples. Error bars indicate standard deviations. **, P < 0.0001; *, P = 0.0034. The same total amount of DNA was used in each transfection by including the pSG5 empty vector in the transfections without the expression construct. (B) Knockdown indicates that KLF2 regulates Myc expression in K562 cells. K562 cells were transfected with Scramble siRNA, KLF2 siRNA (10 nm), or KLF2 siRNA (20 nm). qRT-PCR assay of KLF2 (black bars) and Myc (c-Myc, white bars) mRNAs in transfected cells was performed using cyclophilin A mRNA as an internal standard. The KLF2-to-cyclophilin A mRNA (siRNA Scramble) and Myc-to-cyclophilin A mRNA (siRNA Scramble) ratios were taken as 100%. Error bars show standard deviations. Data are the mean values of 2 independent samples. An asterisk indicates significant difference from siRNA Scramble (P < 0.05).