Fig 8.
(A) Quantitative analyses of mRNA expression in Mycfl/fl; EpoR-Cre and control embryos. Shown is the erythroid-cell-specific GPA expression detected by real-time PCR and normalized to S16 from peripheral blood of Mycfl/fl; EpoR-Cre and control mice at E9.5, E10.5, and E11.5. GPA transcript levels increased significantly in controls, whereas in Mycfl/fl; EpoR-Cre embryos, the levels were virtually unchanged from E9.5. (B) Quantification of embryonic (Ey, βh1, ζ) and adult β-globin expression. qRT-PCR was performed with peripheral blood at E9.5 and E11.5 and normalized to S16. A significant increase in globin gene expression was observed in control embryos from E9.5 to E11.5, while in Mycfl/fl; EpoR-Cre embryos, the expression of none of these genes was increased and expression at E11.5 was comparable to that at E9.5. Data are presented as means ± standard deviations (n ≥ 6). Asterisks indicate significant difference from the control. *, P < 0.02; **, P < 0.01; ***, P < 0.0001.