Fig 16.

(His₁₀)Mus81-(GST)Mms4 suppresses MMS sensitivity of a mus81 mms4 yen1 triple-deletion mutant. The (His₁₀)Mus81-(GST)Mms4 overexpression vectors (pWDH722, pMUS81-MMS4; pWDH596, pmus81-dd-MMS4 [mus81-dd, mus81-D414A, D415A] [21]) were transformed into a mus81Δ mms4Δ yen1Δ triple-deletion strain (WDHY3431). Transformants were analyzed for growth and genotoxin sensitivity. Transformed strains were grown overnight in SD-URA. Overnight cultures were diluted to an OD₆₀₀ of 1, and six 5-fold serial dilutions were plated on SD-URA medium containing 0.0075% MMS or no MMS. Images were taken at day 3.