Table 1.
Yeast strains
| Straina | Genotype |
|---|---|
| W303-RAD5b | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 |
| W303-RAD5b | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 |
| WDHY668 | MATa/α ura3-52/ura3-52 leu2Δ1/leu2Δ1 his3Δ200/his2Δ200 pep4::HIS3/pep4::HIS3 prb1Δ1.6R/prb1Δ1.6R + pWDH722 |
| WDHY1858 | W303-RAD5 MATa mus81::KANMX |
| WDHY1868 | W303-RAD5 MATa MUS81-9MYC::TRP1 |
| WDHY1872 | W303-RAD5 MATa MMS4-9MYC::TRP1 |
| WDHY2128 | W303-RAD5 MATα mms4::TRP1 |
| WDHY2420 | W303-RAD5 MATα MUS81-6HA::TRP1 |
| WDHY2423 | W303-RAD5 MATα MMS4-6HA::TRP1 |
| WDHY2491 | MATa/α ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 |
| WDHY2455 | WDHY2491 MMS4-6HA::TRP1/MMS4-9MYC::TRP1 |
| WDHY2456 | WDHY2491 MUS81-6HA::TRP1/MUS81-9MYC::TRP1 |
| WDHY2492 | WDHY2491 MMS4/MMS4-6HA::TRP1 |
| WDHY2493 | WDHY2491 MUS81/MUS81-6HA::TRP1 |
| WDHY2494 | WDHY2491 MUS81/MUS81-9MYC::TRP1 |
| WDHY2495 | WDHY2491 MMS4/MMS4-9MYC::TRP1 |
| WDHY2612 | W303-RAD5 MATα mus81::KANMX mms4::TRP1 |
| WDHY2733 | W303-RAD5 MATa mus81::KANMX mms4::TRP1 |
| WDHY2783 | W303-RAD5 MATα SLX4-MYC13::KANMX |
| WDHY3267 | WDHY2491 hta1-htb1::LEU2/hta1-htb1::LEU2 MUS81-6HA::TRP1/MUS81-9MYC::TRP1 (hta2-htb2 status unknown), contains pZS145 (pFLAG-HTB1-HTA1::HIS3) |
| WDHY3268 | WDHY2491 hta1-htb1::LEU2/hta1-htb1::LEU2 MUS81/MUS81-6HA::TRP1 (hta2-htb2 status unknown), contains pZS145 (pFLAG-HTB1-HTA1::HIS3) |
| WDHY3269 | WDHY2491 hta1-htb1::LEU2/hta1-htb1::LEU2 MUS81/MUS81-9MYC::TRP1 (hta2-htb2 status unknown), contains pZS145 (pFLAG-HTB1-HTA1::HIS3) |
| WDHY3431 | W303-RAD5 mms4::TRP1 mus81::KANMX yen1::KANMX |
| SFY001c | SLX4-MYC13::KANMX MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 rad5-535 |
| MAO633d | W303 MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 hta1-htb1::LEU2 hta2-htb2 ura3-1::pRG145 UBI4-3HA::URA3 contains pZS145 (pFLAG-HTB1-HTA1::HIS3) |
a
All strains are W303 background with corrected RAD5 and constructed for this study unless otherwise noted.
b
Strains generously provided by Rodney Rothstein.
c
Strain generously provided by John Rouse.
d
Strain generously provided by Mary Ann Osley.