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. 2012 Aug;32(15):3132–3139. doi: 10.1128/MCB.00019-12

Fig 3.

Fig 3

Increased KCa3.1 channel activity and FcεRI-stimulated Ca2+ influx in TRIM27−/− BMMCs. (A) TRIM27+/+ and TRIM27−/− BMMCs were sensitized with anti-DNP IgE, and KCa3.1 channel activity was assessed before (a) and after (b) stimulation with DNP-HSA. (B) Bar graph summary of results in panel A at +40 mV (n = 10 cells) is shown. ∗, P < 0.05 compared to the current in TRIM27+/+ BMMC. (C) Ca2+ influx was assessed in TRIM27+/+ and TRIM27−/− BMMCs as described for Fig. 2D. (D) TRIM27+/+ and TRIM27−/− BMMCs were activated as described above, and the change in membrane potential was determined. ∗, P < 0.05 compared to the membrane potential measured in TRIM27+/+ BMMCs. TRIM27−/− BMMCs were transfected with either a control siRNA or a siRNA to PI3KC2β, and whole-cell patch results (E) or Ca2+ influx (F) was assessed. Also shown in panel E is rescue of KCa3.1 channel activity in PI3KC2β siRNA-transfected cells by the addition of PI3P to the pipette solution. ∗, P < 0.05 compared to the current in TRIM27−/− BMMCs or as indicated.

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