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. 2012 Aug;32(15):3107–3120. doi: 10.1128/MCB.00362-12

Fig 7.

Fig 7

Overexpression of geminin disrupts ORCA-Cdt1 interaction. (A) ORCA immunoprecipitation in U2OS cells transiently overexpressing T7-geminin, followed by ORCA, geminin, Orc2, and Cdt1 immunoblots. Note the loss of ORCA-Cdt1, but not ORCA-Orc2, interaction in cells overexpressing geminin. The arrowheads indicate endogenous and overexpressed geminin. (B) ORCA immunoprecipitation in U2OS cells transiently overexpressing T7 vector (VT), T7-geminin (full-length [FL]), or T7-geminin aa 1 to 160 (G2, which associates only with Cdt1, but not ORCA), followed by geminin, ORCA, Cdt1, and T7 immunoblots. Note that overexpression of full-length geminin, as well as truncated geminin, disrupts ORCA-Cdt1 binding. (C) geminin immunoprecipitation in U2OS cells transiently overexpressing T7-ORCA, followed by geminin, T7, Cdt1, and Orc2 immunoblotting. Note that the overexpression of ORCA does not affect Cdt1-geminin complex formation.