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Claudin-2 is localized at the cell membrane in liver-aggressive breast cancer cells. Following the biotinylation of cell surface molecules, whole-cell lysates were immunoprecipitated with an anti-claudin-2, anti-claudin-4, or antistreptavidin antibody. Immunoblot analyses were performed by using antibodies against claudin-2 (A), claudin-4 (B), or streptavidin (A and B), as indicated.
