Fig 2.
lin-52 mutant larval lethality can be rescued by loss of mip40. (A and B) Two hundred to four hundred fifty progeny per indicated cross were counted. The values listed are the percent viability averages with standard deviations from two independent experiments. mip40EY16520 is a viable null mutation. FM7 and CyO are balancer chromosomes. FM7 is homozygous, viable, and female sterile. CyO is homozygous lethal. (C and D) Immunoblots for Lin-52 and Myb in lin-5293/FM7, Kr-GFP female and lin-5293/Y male embryos and larvae. Tubulin was used as a loading control. (E) Immunoblot of Mip120, Myb, Mip40, and Lin-52 protein in lin-52, mip40 double mutant adult female flies (lin-5293 mip40EY16520) or FM7 control flies.