Fig 3.
Lin-52 is present exclusively in the MMB/dREAM complex and interacts with the complex via its C terminus. (A) Silver-stained SDS-PAGE of eluate from M2 (anti-FLAG IgG) beads after incubation in extract of lin-5293/lin-5293; FLAG–Lin-52/FLAG–Lin-52 embryos grown for 0 to 12 h. The MMB/dREAM subunits were identified by immunoblotting (data not shown). Only the peak fractions are shown. (B) Gel filtration of FLAG–Lin-52-associated proteins. Eluate shown in panel A was fractionated on a Smart Superdex 200 gel filtration column. Sequential fractions were immunoblotted for the proteins indicated at the left. (C) RNA interference was performed in Drosophila Kc cells using double-stranded RNAs targeted against the gene indicated above each lane. After 3 days, cells were harvested and immunoblot analysis was performed with the antibodies indicated on the left.