Fig 4.

Absence of Lin-52 affects the levels of other MMB/dREAM components in vivo. Heat shock-FLP was used to induce FRT-mediated mitotic recombination during the development of heterozygous lin-52⁺/lin-52⁹³ ovarian follicle cells. H2avGFP marks cells bearing the wild-type lin-52⁺ allele. The absence of H2avGFP indicates cells that are homozygous for the lin-52⁹³ null allele. Doubly bright H2avGFP-positive cells represent twin spots bearing two copies of the wild-type lin-52⁺ allele. Ovaries were immunostained with the antibodies indicated on the right of each row. Dotted lines indicate the boundaries of homozygous lin-52⁹³ mutant clones.