Fig 5.

ATF3 does not affect nuclear translocation of AR. PC3 cells were cotransfected with GFP-AR with mCherry-ATF3 or mCherry and then cultured in CSM for 2 days followed by treatments with 1 nM R1881 for 1 h. Cells were then fixed with paraformaldehyde, stained with DAPI (4′,6′-diamidino-2-phenylindole), and observed under a fluorescence microscope (A). At least 300 GFP/mCherry-positive cells were counted for nuclear AR localization (B). Data are depicted as averages ± standard deviations of three determinations.