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ATF3 binds AR at the DBD and LBD regions. (A) Indicated GST-AR fusion proteins were incubated with in vitro-translated ATF3 and subjected to GST pulldown assays. (B) A histidine-tagged AR fragment (aa 537 to 644) containing the DBD was purified by NTA-Ni+-agarose and incubated with immobilized GST-ATF3 or GST for GST pulldown assays. (C) ATF3 was coexpressed with or without FLAG-AR804–919 in PC3 cells by transfections. Cell lysates were subjected to co-IP assays with anti-FLAG antibody.
