Fig 3.

L26-GFP associates with pre-60S ribosomal particles. GFP-tagged L26 was affinity purified with GFP-Trap_A beads from total cellular extracts of rpl26 null cells expressing L26B-eGFP. Wild-type cells were used as an untagged L26 control. RNA was extracted from the pellets obtained after purification (lanes IP) or from an amount of total extracts corresponding to 1/100 of that used for purification (lanes T) and was subjected to Northern analysis of pre- and mature rRNAs. Probes (in parentheses) are described in Fig. S1A and Table S3 in the supplemental material. Signal intensity was measured by phosphorimager scanning; values (below each IP lane) refer to the percentage of each RNA recovered after purification.