Fig 4.

L26 is not essential for growth. (A) The strains BY4741 (wild type), RBY272, a deletant of RPL26A (rpl26aΔ), RBY274, a deletant of RPL26B (rpl26bΔ), and RBY276, an rpl26 null strain (rpl26aΔ rpl26bΔ), were grown in liquid YPD and diluted to an OD₆₀₀ of 0.05. Serial dilutions were spotted onto YPD plates. Plates were incubated at 30 and 37°C for 3 days or at 16°C for 6 days. (B) Total RNA was extracted from cell extract of the strains, separated by gel electrophoresis, transferred to a nylon membrane, and subjected to Northern analysis. The same filter was consecutively hybridized with α-³²P-DNA probes specific for RPL26, RPL35, and ADH1 mRNAs. Mature 25S and 18S rRNAs, which were used as markers to check equal loading, were probed with γ-³²P-labeled oligonucleotides (see Fig. S1A and Table S3 in the supplemental material).