Fig 5.

Absence of L26 results in a very slight deficit of 60S r-subunits. Polysome profiles are shown for the wild-type and the L26-deficient strains described in Fig. 4. Cells were grown in YPD at 30°C and harvested at an OD₆₀₀ of around 0.8. Total extracts were prepared, and 10 A₂₆₀ units of each one were resolved on 7 to 50% sucrose gradients. The A₂₅₄ was continuously monitored. Sedimentation is from left to right. The peaks of free 40S and 60S r-subunits, 80S free couples/monosomes, and polysomes are indicated. Half-mer polysomes are labeled by arrows.