Fig 8.

Depletion of L26 does not significantly affect composition of pre-60S ribosomal particles. (A) TAP-tagged Nop7 was used to purify nuclear pre-60S particles from a conditional GAL::RPL26 strain (JWY9634; GAL::HA-RPL26A rpl26bΔ NOP7-TAP) grown in YPGal (Gal) or shifted to YPD (Glc). Copurifying proteins were separated by SDS-PAGE and stained with silver. (B) Western blotting was used to specifically assay the presence of selected ribosome assembly factors and ribosomal proteins in Nop7-TAP-containing preribosomal particles before and after depletion of L26.