Fig 3.

Phagosomal pH measurement of infected macrophages. Shown are overlaid FACS histograms of pHrodo fluorescence intensities of resting (A) and activated (with IFN-γ plus LPS) (B) murine bone marrow-derived macrophages (BMMΦ) infected with pHrodo-labeled M. tuberculosis WT (solid line), ΔureC (dotted line), or Comp (dashed line) strains and activated BMMΦ infected with pHrodo-labeled WT with (gray line) or without (black line) 200 nM concanamycin (C). Results are representative of two independent experiments.