Fig 4.

Infection profile of M. tuberculosis ΔureC mutant in macrophages. Resting (A) and activated (with IFN-γ plus LPS) (B) murine bone marrow-derived macrophages (BMMΦ) were infected with M. tuberculosis WT, ΔureC, and complemented (Comp) strains at a multiplicity of infection (MOI) of 2. The infected cells were lysed and their bacterial CFU were assessed at 2, 5, and 7 days postinfection (p.i.). (C) Viability of WT and ΔureC mutant under anaerobic conditions at neutral (6.6) or mildly acidic (5.5) pH, in the presence or absence of 3.5 mM urea at day 10. Results are expressed as the means ± standard deviations of triplicates. Results are representative of two independent experiments. *, P = 0.0164.