Fig 5.

Utilization of urea as nitrogen source by M. tuberculosis. WT, ΔureC, and complemented (Comp) strains were grown in minimal media containing 0.2% glycerol only (A) or supplemented with 3.5 mM (B) or 71.4 mM (D) urea. The OD₆₀₀s of the cultures were monitored over a period of 10 days. Arrows indicate time points where the amount of NH₃ present in the culture media was measured (C). Results are representative of two independent experiments.