Table 1.

Spontaneous hydrolysis of urea^a

Urea concn in defined liquid media (mM)	NH3 concn (mg/ml)
0	1.72
3.5	1.97
71.4	5.34

^aLevels of NH₃ in fresh minimal medium without urea, or supplemented with 3.5 or 71.4 mM urea, not inoculated with bacteria, were measured after 10 days of incubation. Results are representative of two independent experiments.