Fig 6.
Nuclear localization of the Cdt subunits in live CHO-K1 cells treated with 10 μg/ml of CdtABLumC and CdtABCLum. (A) Nuclei were isolated from cells exposed to the hybrid toxins for 48 h as described in Materials and Methods. The nuclei were costained with DAPI and Lumio green. In some experiments, cells were treated with MβCD immediately prior to intoxication. The insets show merged DAPI and Lumio green images. The arrowheads mark nuclei positively labeled with Lumio green (green fluorescence) and their corresponding positions in the merged images. The circles show background staining (most likely membrane fragments that do not colocalize with DAPI-stained nuclei). Scale bar = 50 μm. (B) Cells were treated with Cdt containing the various mutated CdtB and CdtC subunits. Nuclei were isolated and labeled as in panel A. Only the merged images are shown.