Fig 6.

Role of genistein and MβCD in invasion of HCT-8 cells by STEC strains 98NK2 and 98NK2ΔfliC. (A) HCT-8 cells were pretreated with 100 μg/ml genistein or vehicle control (water) for 1 h prior to infection. Invasion assays were then performed with STEC strain 98NK2 or 98NK2ΔfliC. Results shown are the means plus SDs from three independent experiments in triplicate. **, P = 0.0056; *, P = 0.0459. (B) HCT-8 cells were pretreated with either 5 or 10 mM MβCD or with vehicle control (DMSO) for 1 h prior to infection. Invasion assays were then performed with strain 98NK2 or 98NK2ΔfliC. Results shown are the means plus SDs from three independent experiments in triplicate. ***, P < 0.0001; **, P = 0.0002; *, P = 0.0456; NS, not significant.