Fig 7.

Association of STEC strain 98NK2 with the lipid raft marker caveolin-1. (A) Uninfected HCT-8 cells in chamber slides were fixed and processed for caveolin-1 labeling as described in Materials and Methods. (B and C) HCT-8 cells were infected with strain 98NK2 for 1 h (B) or 3 h (C) in chamber slides. Slides were fixed and processed for inside-out immunofluorescence and caveolin-1 labeling as described in Materials and Methods. Nuclei are blue (DAPI), extracellular STEC is purple (Alexa Fluor 647), total STEC is green (Alexa Fluor 488), and caveolin-1 is red (Alexa Fluor 594). Bars, 2.5 μm (A and C) and 5 μm (B).