Fig. 4.

α-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid type receptor (AMPAR) subunit protein expression in brain stem preparations from the different treatment groups. Some preparations were either given no stimulation (Naïve) or underwent pseudoconditioning (Ps2) or conditioning (C2) without further treatment. Other preparations were incubated in siRNA alone, followed by conditioning [tGluA4 siRNA, GluA1 siRNA, negative control (NC) siRNA]. Finally, preparations were injected with rescue plasmids into the abducens nerve for transfection of abducens motor neurons, followed by anti-tGluA4 siRNA treatment and C2 (rescue tGluA4 flip; rescue tGluA4 flop). Actin-loading controls are also shown. *Significant differences from Ps2; #significant differences from C2.