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. 2012 Apr 4;108(1):101–111. doi: 10.1152/jn.01097.2011

Fig. 8.

Fig. 8.

Synaptic localization of tGluA4- or tGluA1-containing AMPARs after conditioning or treatment with siRNA and rescue plasmids. A: representative confocal images of abducens motor neurons from the different treatment groups showing punctate staining for synaptophysin (green), GluA1 AMPAR subunits (blue), and GluA4 (red). Double colocalization of GluA1 with synaptophysin (GluA1 + Syn) is cyan, GluA4 with synaptophysin (GluA4 + Syn) is yellow, and triple colocalization of GluA1 and GluA4 with synaptophysin (GluA1 + GluA4 + Syn) is white. Original scale bar = 2 μm. B: quantitative analysis of synaptophysin punctate staining and the colocalization of AMPAR subunits with synaptophysin for the different treatment groups. *Significant differences from Ps2; #significant differences from C2.