Table 1.

Mutations in the tGluA4 siRNA target region for the rescue plasmids (mutations are underlined)

Rescue plasmids for tGluA4 flip and flop dsRNA
Target region of siRNA	AAGTACACATCTGCTCTGACCTACGAT
mutation	AAGTATACATCCGCTCTCACGTACGAT
Rescue plasmids for tGluA4trc1 dsRNA
Target region of siRNA	GGGTCTGTCTGTCTATTGAAATACTAA
mutation	GGGTCGGTCTGTCGATTGGAATACTAA

Plasmids for glutamate receptor A4 subunit and its splice variants from pond turtles, Trachemys scripta elegans, (tGluA4) flip and flop contained 4 nucleotide mutations into the target region of the small-interfering RNA (siRNA), whereas plasmids for a novel, truncated variant of tGluA4 at the C-terminus (tGluA4trc1) contained 3 nucleotide mutations. dsRNA, double-stranded RNA.