. 2012 Apr 4;108(1):101–111. doi: 10.1152/jn.01097.2011

Table 2.

Primers and probes used for real-time RT-PCR

Target	Forward primer	Reverse primer	MGB probe
tGluA4	Total tGluA4 forward `CTATTATGGAAAAAGCGGGACAAA`	Total tGluA4 reverse `CATTGGCTCCTCCATGCATA`	Total tGluA4 `ACATCGTTGCAAACCTGGGATTCAAAGATAT`
tGluA4 flip	tGluA4/tGluA4c flip forward `GGTGAATGTGGAGCCAAGGACT`	tGluA4 flip/flop reverse `TTCAGAAAAGGTCAACTTCATTCGCTT`	tGluA4/tGluA4c flip/flop `CTTCTACATTCTGGTTGGAGGCTTGGGCT`
tGluA4 flop	tGluA4/tGluA4c flop forward `GCAGCGGGGGAGGTGAC`	tGluA4 flip/flop reverse `TTCAGAAAAGGTCAACTTCATTCGCTT`	tGluA4/tGluA4c flip/flop `CTTCTACATTCTGGTTGGAGGCTTGGGCT`
tGluA4c flip	tGluA4/tGluA4c flip forward `GGTGAATGTGGAGCCAAGGACT`	tGluA4c flip/flop reverse `GAGGAAGTTGGATTAAAAGTCTGTGC`	tGluA4/tGluA4c flip/flop `CTTCTACATTCTGGTTGGAGGCTTGGGCT`
tGluA4c flop	tGluA4/tGluA4c flop forward `GCAGCGGGGGAGGTGAC`	tGluA4c flip/flop reverse `GAGGAAGTTGGATTAAAAGTCTGTGC`	tGluA4/tGluA4c flip/flop `CTTCTACATTCTGGTTGGAGGCTTGGGCT`
tGluA4trc1	tGluA4trc1 forward `GGAAATGTTCAGTTTGATCACTATGG`	tGluA4trc1 reverse `TCAATAGACAGACAGACCCTTTCAGATA`	tGluA4trc1 probe `TCGCAGAGTCAACTACACAATGGATG`
Actin	Actin forward `AGGGAAATCGTGCGTGACAT`	Actin reverse `GCGGCAGTGGCCATCTC`	Actin probe `AAGCTGTGCTATGTTGC`

MGB, minor groove binder.