Figure 1.

Protocol of the quantitative single photon emission computed tomography/dual-table autoradiographic (QSPECT/DTARG) method. Iodoamphetamine (IMP) was initially intravenously administered for 1 minute and dynamic cerebral blood flow (CBF) SPECT was started simultaneously and continued for 58 minutes (2 minutes × 14 revolutions, two cycles). Ten minutes after IMP administration, arterial blood sampling was performed and an input function was obtained. After 20 minutes, acetazolamide (Diamox) (17 mg/kg) was intravenously administered for 1 minute. After 30 minutes, the same quantity of IMP as in the first administration was intravenously administered for 1 minute. CBF at rest was measured using data collected from 0 to 28 minutes. CBF after acetazolamide challenge was determined using data from 30 to 58 minutes.