Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Jun;2(6):759–771. doi: 10.1093/nar/2.6.759

Ionic strength effects on the stability and conformation of Penicillium chrysogenum mycophage double stranded RNA.

J P Burnett, B H Frank, R J Douthart
PMCID: PMC343464  PMID: 806900

Abstract

The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stability of the dsRNA compared to DNA of the same base composition was observed over a concentration range of 1.5 times 10- minus 4 M to 4.5 times 10- minus 2 M Na+. At low ionic strengths ([Na+] less than 10- minus 3 M) Tm becomes independent of further decrease in [Na+] unless the dsRNA is exposed to high concentrations of EDTA, suggesting the involvement to tightly bound divalent cation. At relatively high ionic strengths ([Na+] greater than 0.1 M) a postulated A leads to A' ... conformation change occurs.

Full text

PDF
759

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnott S., Fuller W., Hodgson A., Prutton I. Molecular conformations and structure transitions of RNA complementary helices and their possible biological significance. Nature. 1968 Nov 9;220(5167):561–564. doi: 10.1038/220561a0. [DOI] [PubMed] [Google Scholar]
  2. Arnott S., Hukins D. W. Refinement of the structure of B-DNA and implications for the analysis of x-ray diffraction data from fibers of biopolymers. J Mol Biol. 1973 Dec 5;81(2):93–105. doi: 10.1016/0022-2836(73)90182-4. [DOI] [PubMed] [Google Scholar]
  3. Arnott S. The geometry of nucleic acids. Prog Biophys Mol Biol. 1970;21:265–319. doi: 10.1016/0079-6107(70)90027-1. [DOI] [PubMed] [Google Scholar]
  4. Billeter M. A., Weissmann C., Warner R. C. Replication of viral ribonucleic acid. IX. Properties of double-stranded RNA from Escherichia coli infected with bacteriophage MS2. J Mol Biol. 1966 May;17(1):145–173. doi: 10.1016/s0022-2836(66)80101-8. [DOI] [PubMed] [Google Scholar]
  5. Buck K. W., Chain E. B., Himmelweit F. Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA. J Gen Virol. 1971 Aug;12(2):131–139. doi: 10.1099/0022-1317-12-2-131. [DOI] [PubMed] [Google Scholar]
  6. Bush C. A. Optical activity of n-pi transitions in polynucleotides. J Chem Phys. 1970 Nov 1;53(9):3522–3530. doi: 10.1063/1.1674527. [DOI] [PubMed] [Google Scholar]
  7. Carter W. A., De Clercq E. Viral infection and host defense. Science. 1974 Dec 27;186(4170):1172–1178. doi: 10.1126/science.186.4170.1172. [DOI] [PubMed] [Google Scholar]
  8. Cox R. A., Kanagalingam K., Sutherland K. S. Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum. Biochem J. 1970 Dec;120(3):549–558. doi: 10.1042/bj1200549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Douthart R. J., Burnett J. P., Beasley F. W., Frank B. H. Binding of ethidium bromide to double-stranded ribonucleic acid. Biochemistry. 1973 Jan 16;12(2):214–220. doi: 10.1021/bi00726a006. [DOI] [PubMed] [Google Scholar]
  10. Guschlbauer W., Courtois Y., Bové C., Bové J. M. Optical investigations on double stranded ribonucleic acid from turnip yellow mosaic virus. Mol Gen Genet. 1968;103(2):150–158. doi: 10.1007/BF00427142. [DOI] [PubMed] [Google Scholar]
  11. Lemke P. A., Nash C. H. Fungal viruses. Bacteriol Rev. 1974 Mar;38(1):29–56. doi: 10.1128/br.38.1.29-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lemke P. A., Ness T. M. Isolation and characterization of a double-stranded ribonucleic acid from Penicillium chrysogenum. J Virol. 1970 Dec;6(6):813–819. doi: 10.1128/jvi.6.6.813-819.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Petrović S. L., Sumonja B. D., Vasiljević R. B. Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels. Biochem J. 1974 Apr;139(1):157–162. doi: 10.1042/bj1390157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Record M. T., Jr Electrostatic effects on polynucleotide transitions. I. Behavior at neutral pH. Biopolymers. 1967;5(10):975–992. doi: 10.1002/bip.1967.360051010. [DOI] [PubMed] [Google Scholar]
  15. Samejima T., Hashizume H., Imahori K., Fujii I., Miura K. Optical rotatory dispersion and circular dichroism of rice dwarf virus ribonucleic acid. J Mol Biol. 1968 May 28;34(1):39–48. doi: 10.1016/0022-2836(68)90233-7. [DOI] [PubMed] [Google Scholar]
  16. Sato T., Kyogoku Y., Higuchi S., Mitsui Y., Iitaka Y., Tsuboi M., Miura K. I. A preliminay investigation on the molecular structure of rice dwarf virus ribonucleic acid. J Mol Biol. 1966 Mar;16(1):180–190. doi: 10.1016/s0022-2836(66)80271-1. [DOI] [PubMed] [Google Scholar]
  17. Schildkraut C. Dependence of the melting temperature of DNA on salt concentration. Biopolymers. 1965;3(2):195–208. doi: 10.1002/bip.360030207. [DOI] [PubMed] [Google Scholar]
  18. Studdert D. S., Patroni M., Davis R. C. Circular dichroism of DNA: temperature and salt dependence. Biopolymers. 1972;11(4):761–779. doi: 10.1002/bip.1972.360110404. [DOI] [PubMed] [Google Scholar]
  19. TOMITA K. I., RICH A. X-RAY DIFFRACTION INVESTIGATIONS OF COMPLEMENTARY RNA. Nature. 1964 Mar 21;201:1160–1163. doi: 10.1038/2011160a0. [DOI] [PubMed] [Google Scholar]
  20. Tunis-Schneider M. J., Maestre M. F. Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films--a preliminary study. J Mol Biol. 1970 Sep 28;52(3):521–541. doi: 10.1016/0022-2836(70)90417-1. [DOI] [PubMed] [Google Scholar]
  21. Warrington R. C., Hayward C., Kapuler A. M. Conformational studies of reovirus single-stranded RNAs synethesized in vitro. Biochim Biophys Acta. 1973 Dec 7;331(2):231–242. doi: 10.1016/0005-2787(73)90436-x. [DOI] [PubMed] [Google Scholar]
  22. Wood H. A., Bozarth R. F. Properties of viruslike particles of Penicillium chrysogenum: one double-stranded RNA molecule per particle. Virology. 1972 Mar;47(3):604–609. doi: 10.1016/0042-6822(72)90549-1. [DOI] [PubMed] [Google Scholar]
  23. Wood H. A. Viruses with double-stranded RNA genomes. J Gen Virol. 1973 Jun;20(Suppl):61–85. doi: 10.1099/0022-1317-20-Supplement-61. [DOI] [PubMed] [Google Scholar]
  24. Wooley S. Y., Holzwarth G. Optical activity of flow-oriented deoxyribonucleic acid. J Am Chem Soc. 1971 Aug 11;93(16):4066–4068. doi: 10.1021/ja00745a052. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES