Figure 1.
Of all six pro-survival Bcl-2 proteins, Bcl-2 appears the optimal target for ABT-737. (a) MOLT-4 and J16 (Jurkat) T-ALL cell lines that had been transduced to stably express the indicated pro-survival Bcl-2 family members or empty control vector (EV) were treated with a dose range of ABT-737 (μM). Cell death was assessed by PI uptake, 48 h after ABT-737 addition. Gray dashed line indicates the curve fit for the EV dose–response data that is included in every graph for easy reference. Data shown are mean values+S.D. derived from one experiment with triplicate samples and are representative of two to three independent experiments. (b) EC50 values of the dose–response curves in (a) as determined by curve fitting