Figure 6.

Intracellular staining and FACS analysis using Bcl-G-specific mAbs reveals higher Bcl-G expression in CD8⁺ splenic cDCs compared with other DC subsets. To determine the levels of Bcl-G expression in the CD8⁺ and CD8⁻ DC subsets, DC-enriched splenocyte cell suspensions were surface stained with CD11c-FITC and CD8-APC monoclonal antibodies followed by intracellular staining with biotinylated Bcl-G-specific mAbs and incubation with PE-streptavidin (solid line). Cells stained with PE-streptavidin alone were used as negative controls (tinted grey). Samples were analysed using a flow cytometer and representative FACS plots are shown