Figure 6.

FAD inhibits tumor growth in a xenograft model of human colorectal cancer. (a) Tumor growth monitored using Xenogen bioluminescence imaging. Xenografted tumors were established by inoculating luciferase-tagged HCT116 cells into both flanks of athymic nude mice (n=10/group), and their sizes after treatment with FAD at 0 (vehicle), 10, or 15 mg/kg/day were measured by Xenogen bioluminescence imaging at the indicated time points. Representative Xenogen imaging results at days 0 and 28 are shown. (b) Quantitative analysis of the Xenogen bioluminescence imaging data. Average tumor size at the indicated time points as assessed by imaging signal intensities (in photons/second/cm²/steradian). (c) Cell viability was quantified after HCT116 cells were treated with different concentrations of 5-FU for 48 h in the presence or absence of the 3 μM FAD. * and ** indicate P<0.05 and P<0.01, respectively