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Cataract severity in mice evaluated by slit lamp examination at 20 months of age. Each eye was scored on a scale of 0–3, and the mean value of the left and right eye was used as the index of severity for each mouse. Bars shown mean ± SEM for groups of 25–29 female mice or 17–30 male mice. Significance of the trend among four groups of 20-month-old mice (old, Rapa-low, Rapa-mid, and Rapa-high) was evaluated by the nonparametric method of Cuzick (Cuzick, 1985).
