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. Author manuscript; available in PMC: 2012 Sep 6.
Published in final edited form as: IEEE Trans Med Imaging. 2011 Aug 22;31(1):117–130. doi: 10.1109/TMI.2011.2165554

Fig. 1.

Fig. 1

Two examples of neurofilament movement in axons of cultured cortical neurons. The analysis of neurofilament movement in these axons is facilitated by heir relatively low neurofilament content, which results in regions of axon that lack neurofilaments. We refer to these regions as gaps. Fluorescent filaments that move into these gaps can be tracked because of the absence of other neurofilaments. This figure shows frames excerpted from two time-lapse movies. (a)–(f) Filament moves through a gap from left to right (Movie 1) at SNR = 7.5. (g)–(k) A filament moves through a gap from left to right, overlapping with a short pausing filament in frame (j) (Movie 3) at SNR = 5.5. Scale bar = 5 µm. (a) 1.5 min. (b) 2.5 min. (c) 3.5 min. (d) 4.5 min. (e) 5.5 min. (f) 6.5 min. (g) 0 min. (h) 1.5 min. (i) 3 min. (j) 4.5 min. (k) 6 min. (l) 7.5 min.