Comparison of kymograph analysis and the particle filtering tracking algorithms for a rapidly moving neurofilament. These data correspond to frames 118, 119, 129, 130, 132, and 133 of Movie 2 with SNR = 4.1. (a) Kymograph obtained for frames 111 to 181. Note that the neurofilament trajectory is not continuous from frames 118 to 119, 129 to 130, 130 to 131, and 132 to 133 due to the rapid movement and short length of this neurofilament. (b) Tracking results obtained by using SCPF with 50 particles. The bounding boxes measure 10 × 16 pixels throughout. The particle filtering algorithm generates acceptable tracking accuracy.