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. 2012 Jul;194(13):3386–3394. doi: 10.1128/JB.00364-12

Table 2.

PrgX concentrations and plasmid copy numbers in strains used for in vivo prgQ expression studies and in OG1Sp(pCF10)

Straina Plasmid Plasmid copy no./chromosome (approx no. of molecules/cell)b PrgX
No. of molecules/cell Concn (μM)
OG1Sp None 0 0 0
100-5 None 0 715 3.8
OG1Sp pCF10 2.2 (4-5) 696 3.7
OG1Sp pCI3340c 11.2 (4.6) 0 0
a

All strains shown here are derived from OG1Sp.

b

Assuming that the average volume of the cells used in these experiments is 0.31 μM3 and that the average cell contains 2 chromosomes. All copy number determinations were carried out with at least 3 independent cultures, and at least 3 replicate qPCRs carried out on each DNA sample; the standard deviations were all <15% for pCI330 and <20% for pCF10.

c

Plasmid pBK2 and all plasmids used for in vivo analysis of PrgX repression (Fig. 4) used pCI3340 as the cloning vector; we detected no effects of cloned pCF10/prgQ DNA on copy number.