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Binding of ParBc3 to parS-c3, parS-12D, parS-Bsr7, and related or mutated sequences was analyzed by EMSA. Positions 4, 5, and 6 and 11, 12, and 13 are underlined in parS-c3. Underlined bases in the other parS sequences differ from those in parS-c3. The characteristic three-band pattern of ParB-c3 is indicated by arrowheads.
