Table 1.
E. coli strains and plasmids used in this study
| Strain or plasmid name | Genotype | Source |
|---|---|---|
| Strains | ||
| MG1655 | F− prototrophic | C. A. Gross |
| KEI12 | MG1655 except kan+ ymjA+ puuP+A+D+R(3Ala) C+B+E+ pspF+ cat+ | 6 |
| KEI23 | pKEI20/SK212 | This study |
| GM13 | pGM13/SK212 | This study |
| NNE1 | pKJ133/KEI12 | This study |
| NNE3 | pNNB1/KEI12 | This study |
| SH639 | F− Δggt-2 | 22 |
| SK123 | SH639 except ΔpuuDR::kan+ | This study |
| SK167 | SH639 except ΔpuuADR::kan+ | This study |
| SK212 | SH639 except ΔpuuR::kan+ | 6 |
| SK226 | SH639 except ΔpuuA::FRT kan+ FRT | This study |
| Plasmids | ||
| pQE-80L | ColE1 replicon, bla+ lacIq T5p (His)6 | Qiagen |
| pGM05 | pSK103 but puuD+R+ with 266-bp upstream region of puuD | This study |
| pGM06 | pSK103 but puuD+R+ with 214-bp upstream region of puuD | This study |
| pGM07 | pSK103 but puuD+R+ with 193-bp upstream region of puuD | This study |
| pGM08 | pSK103 but puuD+R+ with 156-bp upstream region of puuD | This study |
| pGM09 | pSK103 but puuD+R+ with 100-bp upstream region of puuD | This study |
| pGM13 | pQE-80L but T5p (His)6 puuR(3Ala) | This study |
| pGM14 | pSK103 but puuA+D+R+ with 421-bp upstream region of puuA | This study |
| pGM15 | pSK103 but puuA+D+R+ with 310-bp upstream region of puuA | This study |
| pGM16 | pSK103 but puuA+D+R+ with 229-bp upstream region of puuA | This study |
| pGM17 | pSK103 but puuA+D+R+ with 124-bp upstream region of puuA | This study |
| pGM18 | pSK103 but puuA+D+R+ with 60-bp upstream region of puuA | This study |
| pKEI20 | pQE-80L but T5p (His)6 puuR+ | This study |
| pKJ133 | pUC19 but puuD+R(3Ala) | 6 |
| pKJ134 | pACYC184 but puuP+A+D+R+C+B+E+ | 6 |
| pNNB1 | pUC19 but puuA+D+R(3Ala) with 229-bp upstream region of puuA | This study |
| pNNB2 | pKJ133 but harbors mutation for probe 10 mut1 | This study |
| pNNB3 | pKJ133 but harbors mutation for probe 10 mut2 | This study |
| pNNB4 | pKJ133 but harbors mutation for probe 10 mut3 | This study |
| pNNB5 | pKJ133 but puuA+D+R(3Ala) with 421-bp upstream region of puuA | This study |
| pNNB6 | pSK116 but puuA+D+ΔR with 421-bp upstream region of puuA | This study |
| pSK103 | pUC19 but puuD+R+ | 6 |
| pSK116 | pUC19 but puuD+ΔR | This study |