Fig 1.

EHEC plasmid pO157-2 map and comparison with two other EHEC plasmids. (A) Genetic map of the novel virulence EHEC plasmid pO157-2 generated with CGView (25). Blue block arrows in outer circle denote coding regions in plasmid indicating ORF transcription direction. G+C content is shown in the middle circle, and deviation from the average G+C content (47.71%) is shown in the innermost circle. BLAST comparisons with other two EHEC plasmids are shown in red (pO157) and green (pSFO157). (B) Comparison of whole sequences of plasmid pO157-2 and plasmids pO157 and pSFO157 with MAUVE. Each plasmid genome is laid out in a horizontal track, and homologous segments are indicated in the same color and connected across genomes. Respective scales show the sequence coordinates in base pairs. A colored similarity plot is shown for each genome, the height of which is proportional to the level of sequence identity in that region.