Fig 1.

Sequence of the hlyII promoter with corresponding regulatory elements. (A) hlyII and adjacent genes in B. cereus VKM B-771 and B. thuringiensis VKM B-1555. The transcription start site of hlyII is marked by a bent arrow. The HlyIIR binding site and −10 promoter element are underlined. The sites were mapped by Budarina et al. (9). The putative Fur binding site is shown (21). Numbering is with respect to the hlyII transcription start. (B) Single nucleotide polymorphisms (SNPs) in the Fur binding site. Multiple alignments were constructed using BLAST (word size, 16 bp), searching a 60-bp sequence of the Fur box neighborhood of the PhlyII regions in the B. cereus, B. thuringiensis, B. anthracis, B. mycoides, B. pseudomycoides, B. weihenstephanensis, and B. cytotoxis genomic databases (49 entries). The consensus B. subtilis Fur binding site is shown in bold (16).