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. 2012 Jul;194(13):3327–3335. doi: 10.1128/JB.00199-12

Fig 3.

Fig 3

Fur regulates hlyII-lacZ expression in Bacillus. All strains contain pPH2Z-B771 with PhlyII-lacZ fusion. The results show the means and standard deviations from at least three independent experiments. The significance of results was statistically analyzed using the paired t test (SigmaPlot) (*, P < 0.05; **, P < 0.01). Each experiment was performed at least three times. (A) β-Galactosidase activity in B. thuringiensis VKM B-1555(pPH2Z-B771) under iron-replete (100 μM FeCl3) and iron-depleted (100 μM DPD) conditions (6 h of growth, late exponential phase). (B) Effect of iron supplementation (100 μM FeCl3) on β-galactosidase activity in the wt B. subtilis CU1065 and fur-deficient B. subtilis HB2501. (C) β-Galactosidase activity in the wt B. subtilis CU1065 bearing pFUR-expressed B. cereus fur under iron-replete (100 μM FeCl3) and iron-depleted (100 μM DPD) conditions (2 h of growth, early exponential phase).