Table 1.
Strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Description | Reference or source |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| CU1065 | trpC2 | 41 |
| HB2501 | CU1065 fur::kan | 20 |
| BD170 | BD168 thr-5 trpC2 | E. U. Poluektova |
| EH2 | CU1065 amyE::hlyII | This work |
| EH2R | CU1065 amyE::hlyII-hlyIIR | This work |
| B. cereus VKM B-771 | 35 | |
| B. thuringiensis VKM B-1555 | VKM | |
| E. coli Z85 | thi Δ(lac-proAB) Δ(srl-recA) hsdR supE Tn10(Tcr) (F+ traD proAB lacI ΔM15) | DH5α derivative |
| Plasmids | ||
| pUJ1 | pUC19 with 2.9-kb EcoRI fragment | 35 |
| pHT304-18Z | Promoterless lacZ plasmid | 2 |
| pPH2Z-B771 | hlyII promoter region in pHT304-18Z | This work |
| pEHB | pDG364 derivative | 36 |
| pEH2 | pEHB with hlyII gene | 36 |
| pEH2R | pEHB with hlyII and hlyIIR genes | 36 |
| pFUR6His | fur in pET29(b) | This work |
| pHT01 | MoBiTec | |
| pFUR | B. cereus fur in pHT01 | This work |
| pFUR-Km | Cmr replaced by Kmr in pFUR | This work |
| Oligonucleotides | ||
| hlyIIp-for | GTATCTGGATCCAGGCTGTAATAAGTAAATG | |
| hlyIIp-rev | ACAATGTAGAAGCTTATTAATCTTTATGCC | |
| pHTFur-for | GTCGCTCTAGAATGGAAGAAAGAATTGAACGAATTAAG | |
| pHTFur-rev | ATCGCCCCGGGTTATTTTTCATCCGTTTCATTTTCTT | |
| H2Z-for | AGGAATTTTAGATTATTATGAATGGAAAGG | |
| H2Z-rev | CATTATAACGGACGCTACGGCAACA | |
| Km-NheI-for | CAATTGGCTAGCTTCAACAAACGGGCCAG | |
| Km-SphI-rev | CTTAAGGCATGCCGCCATGACAGCCATG |