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. 2012 Jul;194(13):3407–3416. doi: 10.1128/JB.00113-12

Fig 1.

Fig 1

Construction of H. pylori 26695 mutants deficient in active type II restriction endonucleases. H. pylori wild-type 26695 strain has four active type II restriction endonuclease genes: hpyAII (HP1366), hpyAV (HP0053), hpyAIII (HP0091), and hpyAIV (HP1351) (31). With sacB-mediated counterselection, each of the genes encoding an active restriction endonuclease was sequentially deleted from the genome to finally construct a markerless KO-4 mutant (26695 ΔhpyAII ΔhpyAV ΔhpyAIII ΔhpyAIV [26695-REd]).