Table 1.
Restriction endonuclease | Recognition sequence | No. of restriction sites |
|
---|---|---|---|
cat cassette (1,189 bp) | aphA cassette (1,288 bp) | ||
HpyAII | GAAGA | 2 | 4 |
HpyAV | CCTTC | 2 | 3 |
HpyAIII | GATC | 1 | 7 |
HpyAIV | GANTC | 1 | 7 |
Total | 6 | 21 |
Restriction endonuclease | Recognition sequence | No. of restriction sites |
|
---|---|---|---|
cat cassette (1,189 bp) | aphA cassette (1,288 bp) | ||
HpyAII | GAAGA | 2 | 4 |
HpyAV | CCTTC | 2 | 3 |
HpyAIII | GATC | 1 | 7 |
HpyAIV | GANTC | 1 | 7 |
Total | 6 | 21 |