Skip to main content
. 2012 Jul 18;116(34):18440–18450. doi: 10.1021/jp303267y

Figure 4.

Figure 4

siRNA encapsulated in LNP is fully protected from external RNase. siRNA was either employed in the free form or encapsulated in LNP containing DLinKC2-DMA/DSPC/Chol/PEG-lipid (40/11.5/47.5/1; mol/mol) at an siRNA/lipid ratio of 0.06 (w/w). Encapsulation was performed using the microfluidic mixer as indicated in the Experimental Section. The integrity of the siRNA was challenged with 1 μg/mL bovine pancreatic RNase A. 5% Triton X-100 was added to solubilize the LNP. Gel electrophoresis was performed on 20% native polyacrylamide gel and siRNA visualized by staining with SYBR-Safe.