Fig. 4.

NF-induced transcriptional dynamics within 1 hour after NF-treatment in Medicago. A, Heat map of differentially regulated candidates in wild-type (WT) plants, nfp and dmi3 mutants identified in the transcriptomic data. B, Functional group of genes, which are differentially regulated in the roots of wild-type plants within 1 hour of NF-treatment. The major group of genes, which were differentially regulated, comprise biotic stress/defense, transcriptional factors, transporters, hormonal signaling, protein kinases etc. C, Functional group of genes that are differentially regulated in the roots of nfp mutants within one hour of NF-treatment. The two major groups of genes that were differentially regulated in nfp mutant include transcription factors and genes related to biotic stress/defense response.