Table I. Summary of proteomic and phosphproteomic analyses. NF = Nodulation Factor.

Experiment	1	2	3	4	5	6	7	8	9	10	11	Overall
Treatment	±NF	±NF	±NF	±NF	±NF	±NF	±NF	±NF	±NF	±NF	±NF
Growth	Aeroponic	Hydroponic	Hydroponic	Hydroponic	Plate	Plate	Plate	Plate	Plate	Plate	Plate	–
Cellular Fraction	Whole Cell	Whole Cell	Whole Cell	Whole Cell	Upper Membrane	Lower Membrane	Microsome	Whole Cell	Whole Cell	Upper Membrane	Lower Membrane	–
Genotype	WT, dmi3	WT, dmi3	WT, nfp	WT, nfp	nfp	nfp	WT, nfp, dmi3	WT	WT	WT	WT	–
Biological Replicates	1	1	1	1	3	3	1	3	3	3	3	–
Time Points	N/A	N/A	N/A	N/A	N/A	N/A	N/A	N/A	10/30 min	N/A	N/A	–
Unique Peptidesa	34195	37436	28976	27723	11129	9152	13073	12218	8581	7430	10309	63290
Unique Phosphopeptidesb	5975	9694	8231	5471	2976	4069	2055	4129	2782	1032	1898	15335
Proteins	6315	6983	6290	6521	3996	4071	3956	4256	3603	2865	3604	7739
Phosphoproteins	2392	3076	2800	2382	1351	1706	1000	1807	1320	565	977	3926
Localized Phosphosites	5588	7988	6982	4936	2656	3643	1242	2627	1879	829	1582	13506

^a Unique based on amino acid sequence, does not include phosphopeptides.

^b Unique based on amino acid sequence and number of phosphosites.