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. 2012 May 30;11(9):669–680. doi: 10.1074/mcp.M111.015529

Fig. 1.

Fig. 1.

Probing of the human proteome microarray with PBC and control sera. 26 PBC and 20 control serum samples were diluted 1:1000 and individually incubated with the human proteome microarray, followed by the addition of Cy5-conjugated anti-human IgG antibody. Chips were dried and scanned to acquire images of positive immunoreactions. Representative areas of the images are shown. A, B, and C show that some PBC sera (PBC-584, PBC-746, and PBC-1093) were recognized by HK1 (isoform 1), KLHL12, and ZBTB2, respectively, on the human proteome microarray. The colored box indicates positive candidate autoantigens. D, E, and F show that the control serum samples were negative for the HK1 (isoform 1), KLHL12, and ZBTB2 proteins, respectively.